Diploma in Blood Bank Techniques

No

(in Hours)

·         Collect blood donors medical history and screen donors

·         Draw blood from donor

·         Monitor donor during the donation procedure

·         Screen donated blood for presence of any infection

·         Document, label and store blood donations

·         Check compatibility of blood

HSS / N 2802

HSS / N 2803

HSS / N 2804

HSS / N 2805

HSS / N 2806

HSS / N 2807

·         Work effectively with others

·         Manage work to meet requirements

·         Maintain a safe, healthy and secure environment

·         Practice Code of conduct while performing duties

·         Follow biomedical waste disposal protocols

·         Follow infection control policies and procedures

·         Monitor and assure quality

HSS/ N 9604

HSS/ N 9605

HSS/ N 9606

HSS/ N 9607

HSS/ N 9609

HSS/ N 9610

HSS/ N 9611

(Written and Oral)

·         Candidate should able to Communicate in English.

·         Candidate should able to read and write so that he/she should able to understand the prescription.

·         Candidate should able to use computer for maintaining records for Blood donors medical history.

·         ABO Blood of Group Systems: History, Genetics, ABH antigens, Biochemical Synthesis of blood group antigens, Antigenic sites, weaker variants, Bombay Phenotype, ABO antibodies

·         RH Blood Group System: History, Genetics, Molecular Genetics, Nature of Rh Antigens, Partial D, Week D, other variants of Rh, Rh Null, Rh antibodies, factors influencing Rh immunization, Functional role of Rh antigens

·         Other Blood Group Systems: Lewis, P, Ii, MNSs, Kell, Duffy, Celano, In, Private antigens, Public antigens.

·         Antenatal Serology, Hemolytic disease of the newborn due to ABO Incompatibility, Rh Incompatibility and other allo-antibodies

·         Red cell serology techniques, their advantages and disadvantages, Cell and serum grouping, detection of weak A and B antigens and weak D/Partial D cases, Trouble shooting in red cell serology

·         Pre transfusion testing, Different methods of cross matching, cross matching in special circumstances, emergency cross matching, electronic cross matching

·         Principles of Direct and indirect antiglobulin test, enzyme technique, albumins technique, Detection of blood group antibodies, identification of their Specificity, clinical significance of antibody detection, differentiation between auto and allo-antibodies

·         Gel Technology, Microplate technique

·         Complete hemogram, Different methods of hemoglobin screening/estimation: Copper sulphate, hematology analyzers, Sahli’s, Cyanmethhemoglobin and Hemo-Q methods, Red cell indices

·         Normal erythropoiesis, Leucopoiesis, Formation and function of platelets

·         Classification of anemia, their laboratory diagnosis, Hemoglobinopathy: Beta Thalassemia and Sickle cell disease, G6PD deficiency, polycythemia

·         Autoimmune hemolytic anemia, classification, diagnosis, specificity of autoantibodies

·         Coagulation Mechanism, Hemostasis, laboratory tests for coa, Hemophilia A & B, Platelet disorders,

·         Hematological malignancies

·         Bone marrow transplantation, peripheral stem cells, cord blood stem cells, cord blood banking

·         Antigens : Immunogen, allo-antigen, soluble antigen, Red cell antigen, Epitopes

·         Antibodies: Polyclonal antibodies, development of antibodies, structure of immunoglobulin, characteristics of immunoglobulin

·         Monoclonal antibodies: Hybridoma technology, Human monoclonal antibodies, Applications of MAb

·         Antigen antibody reaction: Antigen concentration, antibody concentration, enhancing media, other factors influencing antigen antibody reaction, Immunoassays: ELISA, IRMA, RIA

·         Cells of immune system: Phagocytic cells, Antigen presenting cells, T cells, T cell subsets, B cells, CD Markers, Flowcytometry for counting T & B cells

·         Autoimmune disorders, their mechanisms,

·         Complement System

·         HLA antigens, HLA antibodies, HLA Serology, Histocompatibility matching: Molecular methods

Microbiology and Biochemistry

·         Groups of Micro organisms, Micro organisms staining techniques

·         Bacteriological media, Pure cultures and cultural characteristics, Bacteria of medical importance,

·         Transfusion transmitted infections, HIV (1+2), HCV, HBV, malaria, syphilis

·         ELISA, rapid and other tests for diagnosis of transfusion transmitted infections

·         Biosafety, Management of Biomedical waste

·         Introduction to Biochemistry, Acid and Base, Buffers and Buffer action, pH, The Beer Law & its application

·         Carbohydrates, proteins, Lipids and Lipoproteins, Red cell membrane integral proteins and lipids, Biochemical estimation of Blood sugar, proteins, Lipid profile and kidney function tests

·         Instrumentation principles: pH meter, colorimeter, Spectrophotometer, Electrophoresis equipment

·         Droplet infection, Measeles, Chicken Pox, Small Pox, Mumps and Influenza

·         Diseases affecting Respiratory System: Tuberculosis, Pneumonia, Asthma, Bronchitis

·         Diseases affecting Gastro Intestinal System: Dysentery, Diorrhea, Typhoid, Cholera, Peptic Ulcer, Liver Diseases (Jaundice, Hepatitis, Cirrhosis, Gastritis, Appendiciitis)

·         Skin Diseases & Venereal Diseases, Scabies, Soriasis, Fungal Infection, Venereal Diseases, Leprosy

·         Blood Anaemia, Leukemia, Bleeding Disorders

·         Urinary Diseases: Glomerulo Nephritis, Pyelo Nephritis

·         Cardiovascular Diseases: Angina Pectoris, Hypertension, Valvular Diseases

·         Nervous System- Polio, Meningitis

·         Viral Diseases : Aids, Rabies

·         Insect-Born Diseases: Malaria, Filariasis

Transfusion Therapy

·         Methods of Health Education

·         Effects of health education

·         Levels & practice of health education

·         Need of Health education to attain positive health

·         Quality control of blood bank raw materials and kits.

·         Quality control of different blood bank Components, sterility test on component.

·         Automation in blood bank

·         Calibration, validation and maintenance of blood bank equipment, QC of blood bank techniques, internal and external QC.

·         Organization of blood bank services, Blood Bank premises and infrastructure, Regional blood transfusion centre and blood storage centres, Blood bank management system

·         Regulations for blood bank operation: Drugs and cosmetics Law, National blood policy, standards in Blood Banking, licensing procedures.

·         Recruitment and training of blood bank personnel, Proficiency test.

·         Blood Bank Accreditation